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Self-Assembly of Electrostatic Cocrystals from Supercharged Fusion Peptides and Protein Cages

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dc.contributor Aalto-yliopisto fi
dc.contributor Aalto University en
dc.contributor.author Korpi, Antti
dc.contributor.author Ma, Chao
dc.contributor.author Liu, Kai
dc.contributor.author Nonappa
dc.contributor.author Herrmann, Andreas
dc.contributor.author Ikkala, Olli
dc.contributor.author Kostiainen, Mauri A.
dc.date.accessioned 2018-10-02T11:29:57Z
dc.date.available 2018-10-02T11:29:57Z
dc.date.issued 2018-03-20
dc.identifier.citation Korpi , A , Ma , C , Liu , K , Nonappa , Herrmann , A , Ikkala , O & Kostiainen , M A 2018 , ' Self-Assembly of Electrostatic Cocrystals from Supercharged Fusion Peptides and Protein Cages ' , ACS Macro Letters , vol. 7 , no. 3 , pp. 318-323 . https://doi.org/10.1021/acsmacrolett.8b00023 en
dc.identifier.issn 2161-1653
dc.identifier.other PURE UUID: 036a77ae-7a7e-4047-9245-279a8b7fe1e1
dc.identifier.other PURE ITEMURL: https://research.aalto.fi/en/publications/036a77ae-7a7e-4047-9245-279a8b7fe1e1
dc.identifier.other PURE LINK: http://www.scopus.com/inward/record.url?scp=85044249580&partnerID=8YFLogxK
dc.identifier.other PURE FILEURL: https://research.aalto.fi/files/28055773/acsmacrolett.8b00023.pdf
dc.identifier.uri https://aaltodoc.aalto.fi/handle/123456789/34106
dc.description.abstract Self-assembly is a convenient process to arrange complex biomolecules into large hierarchically ordered structures. Electrostatic attraction between the building blocks is a particularly interesting driving force for the assembly process, as it is easily tunable and reversible. Large biomolecules with high surface charge density, such as proteins and protein cages, are very promising building blocks due to their uniform size and shape. Assemblies of functional molecules with well-defined nanostructures have wide-ranging applications but are difficult to produce precisely by synthetic methods. Furthermore, obtaining highly ordered structures is an important prerequisite for X-ray structure analysis. Here we show how negatively charged ferritin and viral protein cages can adopt specific cocrystal structures with supercharged cationic polypeptides (SUPs, K72) and their recombinant fusions with green fluorescent protein (GFP-K72). The cage structures and recombinant proteins self-assemble in aqueous solution to large ordered structures, where the structure morphology and size are controlled by the ratio of oppositely charged building blocks and the electrolyte concentration. Both ferritin and viral cages form cocrystals with face centered cubic structure and lattice constants of 14.0 and 28.5 nm, respectively. The crystals are porous and the cationic recombinant proteins occupy the voids between the cages. Such systems resemble naturally occurring occlusion bodies and may serve as protecting agents as well as aid the structure determination of biomolecules by X-ray scattering. en
dc.format.extent 6
dc.format.extent 318-323
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries ACS MACRO LETTERS en
dc.relation.ispartofseries Volume 7, issue 3 en
dc.rights openAccess en
dc.title Self-Assembly of Electrostatic Cocrystals from Supercharged Fusion Peptides and Protein Cages en
dc.type A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä fi
dc.description.version Peer reviewed en
dc.contributor.department Biohybrid Materials
dc.contributor.department University of Groningen
dc.contributor.department Department of Applied Physics
dc.contributor.department Molecular Materials
dc.contributor.department Department of Bioproducts and Biosystems en
dc.identifier.urn URN:NBN:fi:aalto-201810025189
dc.identifier.doi 10.1021/acsmacrolett.8b00023
dc.type.version publishedVersion

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